The effects of pH on DNA methylation state: In vitro and post-mortem brain studies.

  title={The effects of pH on DNA methylation state: In vitro and post-mortem brain studies.},
  author={Carl Ernst and Patrick O McGowan and Vesselina Deleva and Michael J. Meaney and Moshe Szyf and Gustavo Turecki},
  journal={Journal of neuroscience methods},
  volume={174 1},
Assessment of methylation state of DNA extracted from brain is becoming one of the most investigated issues in the study of epigenetics and psychopathology. pH effects in brain are known to affect gene transcription, though pH effects on DNA methylation state are unknown. We demonstrate in vitro using an artificially methylated plasmid that DNA methylation state remains stable, even under extreme pH conditions. Next, using two different genomic regions from human DNA, we assess methylation… CONTINUE READING

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