• DOI:10.1210/ENDO-103-6-1997
• Corpus ID: 38513395

The effects of ouabain on steroid production by rat adrenal cells stimulated by angiotensin II, alpha 1-24 adrenocorticotropin, and potassium.

@article{Braley1978TheEO,
  title={The effects of ouabain on steroid production by rat adrenal cells stimulated by angiotensin II, alpha 1-24 adrenocorticotropin, and potassium.},
  author={L M Braley and Gordon H. Williams},
  journal={Endocrinology},
  year={1978},
  volume={103 6},
  pages={
          1997-2005
        }
}

• L. Braley, G. Williams
• Published 1 December 1978
• Biology, Medicine
• Endocrinology

Aldosterone and corticosterone production by rat adrenal glomerulosa cells was significantly (P < 0.01) increased by the cardiac glycoside, ouabain octahydrate, at a concentration of 10-5 M. Concentrations greater or less had no effect. Ouabain had a biphasic effect when added to cells maximally stimulated by angiotensin II (2.4 × 10-8 M) and αl–24 ACTH (1.4 × 10-10 M); 10-5 M ouabain raised aldosterone output significantly above the already elevated levels (P < 0.05), while 10-3 M ouabain blocked stimulation of aldosterone production by angiotensin II or ACTH completely (P < 0.001). Ouabain at a dose of 10-4 M completely inhibited aldosterone output in response even to large doses of αl–24 ACTH (1.4 × 10-7 M) and significantly reduced glomerulosa cell responses to increasing doses of angiotensin II. In contrast, increasing extracellular potassium concentration caused a dose-dependent reversal of the 10-4 M ouabain block so that at the highest potassium doses used (13 and 18 meq/liter), aldosterone and co...