The effects of ouabain on steroid production by rat adrenal cells stimulated by angiotensin II, alpha 1-24 adrenocorticotropin, and potassium.
@article{Braley1978TheEO, title={The effects of ouabain on steroid production by rat adrenal cells stimulated by angiotensin II, alpha 1-24 adrenocorticotropin, and potassium.}, author={L M Braley and Gordon H. Williams}, journal={Endocrinology}, year={1978}, volume={103 6}, pages={ 1997-2005 } }
Aldosterone and corticosterone production by rat adrenal glomerulosa cells was significantly (P < 0.01) increased by the cardiac glycoside, ouabain octahydrate, at a concentration of 10-5 M. Concentrations greater or less had no effect. Ouabain had a biphasic effect when added to cells maximally stimulated by angiotensin II (2.4 × 10-8 M) and αl–24 ACTH (1.4 × 10-10 M); 10-5 M ouabain raised aldosterone output significantly above the already elevated levels (P < 0.05), while 10-3 M ouabain…
48 Citations
Ouabain increases aldosterone release from bovine adrenal glomerulosa cells: role of renin-angiotensin system.
- BiologyThe American journal of physiology
- 1996
These findings together with a stimulation of renin release by ouabain indicate that angiotens in II generated by the adrenal cell renin-angiotensin system in the presence of exogenous serum or exogenous angiotENSinogen is necessary for the ou abain-induced stimulation of aldosterone release.
Ouabain is a potent inhibitor of aldosterone secretion and angiotensin action in the human adrenal.
- Biology, MedicineThe Journal of clinical endocrinology and metabolism
- 1996
It is suggested that nanomolar levels of ouabain can reduce aldosterone secretion from human zona glomerulosa cells, as well as inhibiting Na,K-adenosine triphosphatases and could regulate ald testosterone secretion.
Effects of ouabain and potassium on protein synthesis and angiotensin-stimulated aldosterone synthesis in bovine adrenal glomerulosa cells.
- Biology, ChemistryEndocrinology
- 1986
Since protein synthesis is necessary for angiotensin II stimulation of cholesterol side-chain cleavage, ouabain depresses that step, pregnenolone synthesis, and thus, aldosterone synthesis, in bovine adrenal glomerulosa cells, and these data fit a model in which ou abain causes loss of cell potassium, which, in turn, depresses protein synthesis.
Role of Ca2+ in response of adrenal glomerulosa cells to angiotensin II, ACTH, K+, and ouabain.
- Biology, MedicineThe American journal of physiology
- 1981
The results suggest that Ca2+ mediates the effects of angiotensin II, ACTH, K+ and Na+-K+-ATPase inhibition on aldosterone biosynthesis, and may be the final common intracellular messenger of most ald testosterone secretagogues.
Effect of sodium deficiency on beta-melanocyte-stimulating hormone stimulation of aldosterone in isolated rat adrenal cells.
- Biology, MedicineEndocrinology
- 1983
Sodium deficiency enhances the sensitivity of the adrenal glomerulosa cells tobeta MSH, causing significant stimulation of aldosterone production by doses of beta MSH within the physiological range, and data suggest that betaMSH or peptides containing Beta MSH may play a role in the regulation of a Aldosterone secretion during sodium deficiency in the rat.
Interactions between ouabain, atrial natriuretic peptide, angiotensin-II and potassium: effects on rat zona glomerulosa aldosterone production.
- Biology, MedicineLife sciences
- 1998
Regulation of aldosterone secretion by a new aldosterone stimulating factor.
- Biology, MedicineJapanese circulation journal
- 1982
The present study suggests ASF shares a common rate-limiting final pathway of steroidogenesis, which may be the step between corticosterone to aldosterone, with ACTH, A II and beta-LPH.
Endothelin-1 stimulation of aldosterone and zona glomerulosa ouabain-sensitive sodium/potassium-ATPase
- Biology, MedicineThe Journal of Steroid Biochemistry and Molecular Biology
- 1994
Potassium fluxes in bovine adrenal cells during adrenocorticotropin stimulation.
- Biology, MedicineEndocrinology
- 1985
Changes in flux were measured in isolated bovine zona fasciculata/reticularis cells and increases in cortisol synthesis by ouabain-treated cells in response to angiotensin II and ACTH were proportional to decreases in potassium influx.
Effects of extracellular potassium and 4-aminopyridine on corticosteroid secretion
- Biology, MedicineMolecular and Cellular Endocrinology
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