The effects of nitrous oxide on the secretory activity of pro-opiomelanocortin peptides from basal hypothalamic cells attached to cytodex beads in a superfusion in vitro system

@article{Ziga1987TheEO,
  title={The effects of nitrous oxide on the secretory activity of pro-opiomelanocortin peptides from basal hypothalamic cells attached to cytodex beads in a superfusion in vitro system},
  author={Jaime Z{\'u}{\~n}iga and Shirley A. Joseph and Karl M. Knigge},
  journal={Brain Research},
  year={1987},
  volume={420},
  pages={66-72}
}
Dispersed cells from adult rat basal hypothalami, attached to Cytodex-3 microcarrier beads, were placed in a column and superfused with aerated high glucose media or media enriched with variable concentrations of nitrous oxide with oxygen. beta-Endorphin and alpha-MSH content was measured in the effluent collected during superfusion and demonstrated a near constant baseline release. Nitrous oxide, 60% (P less than 0.025) and 80% (P less than 0.02), caused significant increases in release of… CONTINUE READING
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