The effects of isolation on chondrocyte gene expression.

  title={The effects of isolation on chondrocyte gene expression.},
  author={Danika M Hayman and Todd J Blumberg and C. Corey Scott and Kyriacos A. Athanasiou},
  journal={Tissue engineering},
  volume={12 9},
Tissue engineering of articular cartilage usually requires the isolation and culture of chondrocytes. Previous studies have suggested that enzymatic isolation may alter the metabolic activity and growth rate of chondrocytes. This study examined the effects of 4 common isolation protocols on chondrocyte gene expression, morphology, and total cell yield immediately following the digest (t = 0) and after 2 culture periods (24 h and 1 week). Cartilage explants were digested using 1 of 4 protocols… CONTINUE READING


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