The effects of internal primer-template mismatches on RT-PCR: HIV-1 model studies.

@article{Christopherson1997TheEO,
  title={The effects of internal primer-template mismatches on RT-PCR: HIV-1 model studies.},
  author={Cindy D Christopherson and John Sninsky and S. Kwok},
  journal={Nucleic acids research},
  year={1997},
  volume={25 3},
  pages={654-8}
}
We investigated the effects of internal primer-template mismatches on the efficiency of reverse transcription and PCR amplification. As models, RNA transcripts representative of different HIV-1 group M subtypes were evaluated with a previously described gag primer pair system. We observed that the presence of two to four mismatches in the primer-template duplexes did not have a significant effect on RT-PCR. However, the presence of five and six mismatches with the 28 and 30 base primers reduced… CONTINUE READING
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