The effects of bombesin on polyphosphoinositide and calcium metabolism in Swiss 3T3 cells.

  title={The effects of bombesin on polyphosphoinositide and calcium metabolism in Swiss 3T3 cells.},
  author={N. Takuwa and Y. Takuwa and W. Bollag and H. Rasmussen},
  journal={The Journal of biological chemistry},
  volume={262 1},
Bombesin, a peptide mitogen for a variety of cell types, acts as a typical Ca2+-mobilizing hormone in Swiss 3T3 fibroblasts. At its mitogenic concentrations (1-25 nM), bombesin stimulates polyphosphoinositide turnover, i.e. breakdown of phosphatidylinositol 4,5-bisphosphate and a concomitant increase in inositol phosphates in a time- and dose-dependent manner. In particular, bombesin induces an initial transient increase in inositol 1,4,5-trisphosphate concentration, followed by an increase in… Expand
Mechanism of action of bombesin on amylase secretion. Evidence for a Ca2+-independent pathway.
  • R. Bruzzone
  • Biology, Medicine
  • European journal of biochemistry
  • 1989
Results indicate that bombesin, an agonist thought to activate secretion mainly through mobilization of Ca2+ from intracellular stores, elicits amylase release at low concentrations, independently of a concomitant rise in cytosolic free Ca2+. Expand
Homologous desensitization of bombesin‐induced increases in intracellular Ca2+ in quiescent swiss 3T3 cells involves a protein kinase C‐independent mechanism
It is concluded that acute homologous desensitization of Ca2+ responses induced by bombesin occurs through a PKC‐independent mechanism. Expand
Stimulation of calcium mobilization but not proliferation by bombesin and tachykinin neuropeptides in human small cell lung cancer cells.
The results indicate that bombesin/gastrin-releasing peptide and other calcium mobilizing peptides do not always act as a growth factor in SCLC cells, and that theBombesin antagonist could inhibit growth of SclC cells through a mechanism other than bombsin antagonism. Expand
Endothelin-1 activates phospholipase C and mobilizes Ca2+ from extra- and intracellular pools in osteoblastic cells.
The results demonstrate that ET activates the inositol lipid signaling pathway and induces mobilization of Ca2+ from both extra- and intracellular pools and activation of protein kinase C in osteoblastic MC3T3-E1 cells. Expand
Bombesin stimulation of mitogenesis. Specific receptors, signal transduction, and early events.
  • E. Rozengurt
  • Biology, Medicine
  • The American review of respiratory disease
  • 1990
Studies using membrane preparations and permeabilized 3T3 cells indicate that G proteins play a role in the transduction of the mitogenic signal triggered by the binding of bombesin to its receptor. Expand
Mitogenesis in response to PDGF and bombesin abolished by microinjection of antibody to PIP2.
The findings strongly suggest that PIP2 breakdown is crucial in the elicitation and sustaining of cell proliferation induced by some types of mitogens such as platelet-derived growth factor and bombesin. Expand
Activation of inositol phospholipid signaling and Ca2+ efflux in human breast cancer cells by bombesin.
Data implicate a functional role for BRPs in human breast cancer by studying their effect on phospholipid hydrolysis, 45Ca2+ efflux, and cell growth in thehuman breast cancer cell line MCF-7 and activation of putative mitogenic signaling pathways. Expand
Bombesin enhancement of cAMP accumulation in swiss 3T3 cells: Evidence of a dual mechanism of action
The additive nature of PBt2‐mediated down‐regulation and treatment with indomethacin suggests that activation of protein kinase C and the release of E type prostaglandins provide two distinct pathways involved in the enhancement of cAMP accumulation by bombesin. Expand
Characterization of the Bombesin/Gastrin-Releasing Peptide Receptor in Swiss 3T3 Fibroblasts
This chapter describes some of the techniques that are most relevant to achieve the specificities of the G protein–receptor relationship and demonstrates that bombesin acts through signal transduction pathways, in which effector stimulation is tightly coupled to the activation of a G protein. Expand
Bombesin stimulation of c-fos expression and mitogenesis in Swiss 3T3 cells: the role of prostaglandin E2-mediated cyclic AMP accumulation.
It is concluded that cAMP plays a regulatory role in c-fos induction and mitogenesis in Swiss 3T3 cells treated with bombesin and the inhibitory effect of indomethacin was reversed in the presence of forskolin, a direct activator of adenylate cyclase. Expand