The effects of benzyladenine, gibberellin and paclobutrazol on organogenesis in Cymbidium species

  title={The effects of benzyladenine, gibberellin and paclobutrazol on organogenesis in Cymbidium species},
  author={Kazuhiko Shimasaki and Jiro Tsuneisi and Yasufumi Fukumoto},
  journal={Shokubutsu Kojo Gakkaishi},
Addition of paclobutrazol (PAC) to modified MS medium induced prptocorm-like body (PLB) formation from rhizome segment cultures of Cyrnbidium kanran Makino (terrestrial type). The combination of BA with PAC reduced the number of PLB. Application of BA induced shoot (protocorm-like pseudobulb with short leaves) without root. Single addition of 10μMBA had a significant effect on induction and increased the number of shoots. PAC in culture medium inhibited BA-induced leaf formation from rhizome… 
1 Citations
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The modified MS medium containing lesser amounts of potassium nitrate and ammonium nitrate than those of the original MS media was optimal for the production of plantlets from rhizomes of C. kanran without addition of auxin and cytokinin.
Interactive Effects between Cytokinin and Ethephon on Shoot Formation in Rhizome Cultures of Cymbidium kanran Makino
Rhizomes of Cymbidium kanran were cultured in liquid Murashige and Skoog (MS) media supplemented with zeatin, BA and/or ethephon to reduce the ethylene evolution from rhizome cultures and to inhibit branching of rhizomes.
An Anatomical Study of Rhizome Bud Formation Induced by Paclobutrazol and Adventitious Root Formation in In Vitro Cultures of Lapageria rosea (Ruiz et Pav.)
An anatomical study was made of bud dimorphism in in vitro shoot cultures of Lapageria rosea cv. Nashcourt, utilizing the presence and absence of the gibberellin-biosynthesis inhibitor paclobutrazol
Rhizome Formation in Cymbidium goeringii Reichenbach fil. and Cymbidium kanran Makino in Shoot-Tip Culture
Culturing of shoot-tips of Cymbidium goeringii at intervals of 2 months showed that rhizomes could be formed at any time throughout the year, however, rhizome formation depended on the starting time of cultures and the culture media.
Organogenesis in meristem cultures of Cymbidiums. I
Increase in fresh weight and branching of protocorm were considerably affected by sucrose concentration of the culture medium, and the most abundant growth was observed at 4% in both C. pumilum and C. goeringii.
Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh.
It is shown that GA levels play a role in regulating shoot bud regeneration from callus, and it is suggested that variation in endogenous GA levels or responsivity may account for a major component of the genetic variation inshoot bud regeneration frequency described in other species.
Induction of adventitious shoot , rhizome - derived protocorm - like bodies and abnormal shoot - tip aggregations from rhizome segments of Pogonia japon
  • 1998