The effects of amino acids and ammonium on the growth of plant cells in suspension culture.

@article{Gamborg1970TheEO,
  title={The effects of amino acids and ammonium on the growth of plant cells in suspension culture.},
  author={Oluf L. Gamborg},
  journal={Plant physiology},
  year={1970},
  volume={45 4},
  pages={372-5}
}
A suspension culture of soybean (Glycine max L.) was grown on a defined medium in which the nitrogen sources were nitrate (25 mM) and ammonium (2 mM). The cells did not grow on nitrate unless the medium was supplemented with ammonium or glutamine. The l- and d-isomers of 12 amino acids tested singly could not replace ammonium. Most amino acids (4 mM) inhibited growth when the cells were cultured on nitrate and ammonium. Cells from five other plants (Reseda luteoli L.; Triticum monococcum L… CONTINUE READING

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Studies on the growth requirements and growth measurements of cell cultures of Haplopappus gracilis

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