The effects of a 45 000 molecular weight protein from unfertilized sea urchin eggs and its 1:1 actin complex on actin filaments

@article{Coluccio1986TheEO,
  title={The effects of a 45 000 molecular weight protein from unfertilized sea urchin eggs and its 1:1 actin complex on actin filaments},
  author={Lynne M. Coluccio and Perry A. Sedlar and Joseph J. Bryan},
  journal={Journal of Muscle Research & Cell Motility},
  year={1986},
  volume={7},
  pages={133-141}
}
A 45 kDa actin-binding protein (SU45) has been isolated previously from egg extracts of the Hawaiian sea urchinTripneustes gratilla by DEAE Sephacel, Sephadex G-75 and hydroxyapatite chromatography. Using pyrene-labelled rabbit skeletal muscle actin, we have found that when SU45 is added to actin in the presence of calcium and the salt concentration is increased, the initial rate of actin assembly is accelerated. Moreover, the final polymer concentration is reduced indicating that SU45 caps the… CONTINUE READING

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A 45000molwt p ro t e i ~ ac t i n complex from unfertil ized sea urchin eggs affects assembly propert ies of actin

H. HOSOYA, I. MABUCHI
J . Cell Biol . • 1984

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