The effects of RNA degradation enzymes on antisense RNAI controlling ColE2 plasmid copy number.

@article{Nishio2008TheEO,
  title={The effects of RNA degradation enzymes on antisense RNAI controlling ColE2 plasmid copy number.},
  author={Shin-Ya Nishio and Tateo Itoh},
  journal={Plasmid},
  year={2008},
  volume={60 3},
  pages={174-80}
}
Replication of the ColE2 plasmid requires a plasmid-coded initiator protein (Rep). Rep expression is controlled by antisense RNA (RNAI), which prevents the Rep mRNA translation. In this paper, we examined the effects of RNA degradation enzymes on the degradation pathways of RNAI of the ColE2 plasmid. In the DeltapcnB strain lacking the poly(A) polymerase I (PAP I) the RNAI degradation intermediate (RNAI(*)) accumulates much more than that in the wt strain. RNAI(*) is produced by the RNase E… CONTINUE READING