The effect of weak inertia in rotating high-aspect-ratio vessel bioreactors

  title={The effect of weak inertia in rotating high-aspect-ratio vessel bioreactors},
  author={Mohit P. Dalwadi and Stephen Jonathan Chapman and James M. Oliver and Sarah L. Waters},
  journal={Journal of Fluid Mechanics},
  pages={674 - 720}
One method to grow artificial body tissue is to place a porous scaffold seeded with cells, known as a tissue construct, into a rotating bioreactor filled with a nutrient-rich fluid. The flow within the bioreactor is affected by the movement of the construct relative to the bioreactor which, in turn, is affected by the hydrodynamical and gravitational forces the construct experiences. The construct motion is thus coupled to the flow within the bioreactor. Over the time scale of a few hours, the… 
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  • Biology
    Biotechnology and bioengineering
  • 2003
This analysis deals with advances in tissue‐engineering models and computational methods as well as with novel results on the relative importance of “controlling forces” in the growth of organic constructs, focused on the rotary culture system.

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A validated model of GAG deposition, cell distribution, and growth of tissue engineered cartilage cultured in a rotating bioreactor

Validation of the model predictive capability suggests that the model successfully describes the interplay of several simultaneous processes carried out during in vitro cartilage tissue regeneration and indicates that this approach could also be attractive for application in other tissue engineering systems.

Microgravity tissue engineering

Constructs grown in simulated microgravity contained the highest fractions of total regenerated tissue and of GAG, the component required for cartilage to withstand compressive force, as a percent of construct dry weight.