The effect of trifluoroethanol on tyrosinase activity and conformation: inhibition kinetics and computational simulations.

@article{L2010TheEO,
  title={The effect of trifluoroethanol on tyrosinase activity and conformation: inhibition kinetics and computational simulations.},
  author={Zhi-Rong L{\"u} and Long Shi and Jun Wang and Daeui Park and Jong Bhak and Jun-Mo Yang and Y C Park and Hong-wei Zhou and Fei Zou},
  journal={Applied biochemistry and biotechnology},
  year={2010},
  volume={160 7},
  pages={1896-908}
}
We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of tyrosinase. TFE increased the degree of secondary structure of tyrosinase, which directly resulted in enzyme inactivation. A reciprocal study showed that TFE inhibited tyrosinase in a slope-parabolic mixed-type inhibition manner (K (I) = 0.5 +/- 0.096 M). Time-interval kinetic studies showed that the inhibition was best described as first order with biphasic processes. Intrinsic and 1… CONTINUE READING