The effect of temperature on the proteome of recombinant Pichia pastoris.

@article{Dragosits2009TheEO,
  title={The effect of temperature on the proteome of recombinant Pichia pastoris.},
  author={Martin Dragosits and Johannes Stadlmann and Joan Albiol and Kristin Baumann and Michael Maurer and Brigitte Gasser and Michael G Sauer and Friedrich Altmann and Pau Ferrer and Diethard Mattanovich},
  journal={Journal of proteome research},
  year={2009},
  volume={8 3},
  pages={1380-92}
}
The impact of environmental factors on the productivity of yeast cells is poorly investigated so far. Therefore, it is a major concern to improve the understanding of cellular physiology of microbial protein production hosts, including the methylotrophic yeast Pichia pastoris. Two-Dimensional Fluorescence Difference Gel electrophoresis and protein identification via mass spectrometry were applied to analyze the impact of cultivation temperature on the physiology of a heterologous protein… CONTINUE READING
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