The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.

@article{Wu1991TheEO,
  title={The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.},
  author={D Y Wu and L Ugozzoli and Bijay K Pal and Ji Qian and Robert B. Wallace},
  journal={DNA and cell biology},
  year={1991},
  volume={10 3},
  pages={233-8}
}
The polymerase chain reaction (PCR) is most effectively performed using a thermostable DNA polymerase such as that isolated from Thermus aquaticus. Since temperature and oligonucleotide length are known to control the specificity of oligonucleotide hybridization, we have investigated the effect of oligonucleotide length, base composition, and the annealing temperature on the specificity and efficiency of amplification by the PCR. Generally, the specificity of PCR is controlled by the length of… CONTINUE READING
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