The effect of swainsonine and castanospermine on the sulfation of the oligosaccharide chains of N-linked glycoproteins.

@article{Merkle1985TheEO,
  title={The effect of swainsonine and castanospermine on the sulfation of the oligosaccharide chains of N-linked glycoproteins.},
  author={Roberta K Merkle and Alan D Elbein and Aviad Heifetz},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 2},
  pages={1083-9}
}
MDCK (Madin-Darby canine kidney) cells infected with the NWS strain of influenza virus incorporate 35SO4 into complex types of oligosaccharides of the N-linked glycoproteins. On the other hand, when these virus-infected MDCK cells are incubated in the presence of swainsonine, an inhibitor of the processing mannosidase II, approximately 40-80% of the total [35S]glycopeptides were of the hybrid types of structures. Thus, these sulfated, hybrid types of glycopeptides were completely susceptible to… CONTINUE READING