The effect of substrate cycling on the ATP yield of sperm glycolysis.

@article{Hammerstedt1983TheEO,
  title={The effect of substrate cycling on the ATP yield of sperm glycolysis.},
  author={Roy H Hammerstedt and Henry A. Lardy},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 14},
  pages={8759-68}
}
The conversion of glucose to lactate via the Embden-Meyerhof pathway yields a maximum of 2 mol of ATP per mol of glucose degraded with lesser amounts of ATP obtained if metabolic intermediates are removed for biosynthesis or if substrate cycling occurs during carbohydrate degradation. Bull sperm are an ideal test system for a quantitative estimate of ATP yield because they contain enzymes necessary for potential substrate cycling and do not carry out significant biosynthesis. Experiments… CONTINUE READING