There were performed experimental study on the effect of Al(OH)3, 16,16dmPGE2, indomethacin, verapamil and calcium ionophore A 23157 on alcohol-induced injury of isolated gastric mucosal cells of rat. In the first step a dose dependent alcohol injury of isolated gastric mucosal cells was shown. The most susceptible cells to alcohol damage were parietal cells. 16,16dmPGE2 and verapamil (calcium channel inhibitor), except Al(OH)3, were cytoprotective--they protected surface epithelial cells against alcohol. Indomethacin and calcium ionophore A 23157 (increases the calcium influx into the cell) aggravated the alcohol damage to gastric cells. The results show important role of prostaglandin and calcium ions in the mechanism of gastric mucosal cell damage and indicate the lack of direct influence of antacids on these cells in vitro, in conditions independent of microcirculation and neurohormonal factors.