The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg

@article{Haima1987TheEO,
  title={The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg},
  author={Peter Haima and Sierd Bron and Gerard A. Venema},
  journal={Molecular and General Genetics MGG},
  year={1987},
  volume={209},
  pages={335-342}
}
Using the bifunctional cloning vehicle pHP13, which carries the replication functions of the cryptic Bacillus subtilis plasmid pTA1060, the effects of BsuM restriction on the efficiency of shotgun cloning of heterologous Escherichia coli DNA were studied. In a restriction-deficient but modification-proficient mutant of B. subtilis, clones were obtained at a high frequency, comparable to frequencies normally obtained in E. coli (104 clones per μg target DNA). Large inserts were relatively… CONTINUE READING