The effect of protein kinase C activation on colonic epithelial cellular integrity.

@article{Tepperman2000TheEO,
  title={The effect of protein kinase C activation on colonic epithelial cellular integrity.},
  author={Barry L. Tepperman and Brian Soper and Qing Chang and James F. Brown and Candice Wakulich},
  journal={European journal of pharmacology},
  year={2000},
  volume={389 2-3},
  pages={131-40}
}
We have investigated whether activation of protein kinase C has a direct cytotoxic effect on colonic mucosal epithelial cells and whether oxidant-induced damage to colonocytes is mediated by activation of cellular protein kinase C. Incubation of freshly harvested cells from rat colon with the protein kinase C activator, phorbol 12-myristate, resulted in a concentration-dependent increase in the extent of cell injury. Phorbol 12-myristate acetate (0.1-10 microM) also increased cellular protein… CONTINUE READING