The effect of phospholipase A(2) immobilization upon calcium interaction: a kinetic study.

Abstract

In this work we studied the effect of Ca(2+) on the ability of immobilized PLA(2) to hydrolyze phospholipid substrates either in aggregate or monomeric forms. We use a kinetic methodology for the determination of dissociation constants of soluble and immobilized PLA(2)-Ca(2+) complexes. This approach allows us to obtain the values of the dissociation… (More)

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Cite this paper

@article{Madoery1999TheEO, title={The effect of phospholipase A(2) immobilization upon calcium interaction: a kinetic study.}, author={Ricardo Rom{\'a}n Madoery and C G Gattone and Gerardo Daniel Fidelio}, journal={Journal of biochemistry}, year={1999}, volume={126 6}, pages={1060-6} }