The effect of pH upon human transferrin: selective labelling of the two iron-binding sites.

@article{Lestas1976TheEO,
  title={The effect of pH upon human transferrin: selective labelling of the two iron-binding sites.},
  author={A N Lestas},
  journal={British journal of haematology},
  year={1976},
  volume={32 3},
  pages={341-50}
}
  • A N Lestas
  • Published 1976 in British journal of haematology
The influence of pH changes upon the iron-binding properties of transferrin was investigated in the absence of chelating agents. The effects were demonstrated by spectrophotometry, gel filtration, and by studies of the intermolecular transfer of 59Fe from transferrin to conalbumin. At pH values below 6.7, diferric transferrin readily loses iron. The monoferric molecule, which is relatively resistant to acid dissociation, is preferentially formed. A temporary reduction of pH provides a simple… CONTINUE READING