The effect of pH, bile and calcium on the adhesion ability of probiotic enterococci of animal origin to the porcine jejunal epithelial cell line IPEC-J2.

@article{Marcikov2010TheEO,
  title={The effect of pH, bile and calcium on the adhesion ability of probiotic enterococci of animal origin to the porcine jejunal epithelial cell line IPEC-J2.},
  author={Miroslava Marciň{\'a}kov{\'a} and Trine Dan\o Klingberg and Andrea Laukov{\'a} and Birgitte Bj\orn Budde},
  journal={Anaerobe},
  year={2010},
  volume={16 2},
  pages={120-4}
}
Examination of adhesion ability using a quantitative assay based on radiolabelled bacteria showed that 10 Enterococcus strains exhibited adhesion ability from 2 to 4%. Enterococcus faecium EF2019 (isolate from rabbit faeces, deponed to Czech Culture of Microorganisms in Brno, CCM 7420) showed the highest adhesion ability (4.0+/-0.4%). With regard to survival, all strains displayed good resistance towards 0.3% oxgall and HCl (pH 3.0). Pretreatment of strains with HCl (pH 3.0) significantly… CONTINUE READING