The effect of molecular crowding with nucleotide length and cosolute structure on DNA duplex stability.

@article{Nakano2004TheEO,
  title={The effect of molecular crowding with nucleotide length and cosolute structure on DNA duplex stability.},
  author={Shu-Ichi Nakano and Hisae Tateishi Karimata and Tatsuo Ohmichi and Junji Kawakami and Naoki Sugimoto},
  journal={Journal of the American Chemical Society},
  year={2004},
  volume={126 44},
  pages={14330-1}
}
The thermodynamics of DNA duplex structures in the presence of high concentrations of cosolutes in solution were investigated to discern nucleic acid structures and functions in living cells. In the presence of ethylene glycol (EG) and poly(ethylene glycol) (PEG) (MW = 200-8000), the stability of the oligomer DNA duplexes with differing nucleotide length varied, depending on the nucleotide length as well as the size of PEG. It was also revealed that the decrease of water activity is the primary… CONTINUE READING