The effect of membrane potential on the mammalian sodium-potassium pump reconstituted into phospholipid vesicles.

@article{Goldshlegger1987TheEO,
  title={The effect of membrane potential on the mammalian sodium-potassium pump reconstituted into phospholipid vesicles.},
  author={R Goldshlegger and Steven J D Karlish and Ada Rephaeli and Wilfred Donald Stein},
  journal={The Journal of physiology},
  year={1987},
  volume={387},
  pages={331-55}
}
1. We have studied effects of electrical diffusion potentials on active Na+-K+ exchange in phospholipid vesicles reconstituted with pig kidney Na+, K+-ATPase. 2. Diffusion potentials, negative inside, were established using outwardly directed K+ gradients plus valinomycin or Li+ gradients plus a Li+ ionophore, AS701. Measurement of fluorescence changes of the carbocyanine dye DiS-C3-(5) showed that the ionophores generated potentials of the expected orientation and of sufficient stability for… CONTINUE READING
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