Human mononuclear cell supernatants were obtained by incubating 3 X 10(6) cells per ml of Dulbecco's modified Eagles medium at 37 degrees C for 24 h or 48 h, either in presence or absence of phytohaemagglutinin. After removal of intact cells, the supernatants were dialysed and diluted (1:1, 1:2, 1:5, 1:10) again using the above medium. The diluted… (More)
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