The effect of liposomally conjugated methotrexate upon mediator release from human peripheral blood monocytes.

Abstract

The ability of methotrexate (MTX) and a liposomal preparation containing a lipophilic conjugate of methotrexate (MTX-LIPO) to modulate pro-inflammatory mediator release from lipopolysaccharide-stimulated human peripheral blood monocytes was investigated. At non-toxic concentrations MTX-LIPO (10 micrograms MTX per 250 micrograms lipid) was a potent inhibitor of both IL-1 beta and TNF release resulting in 70.07 +/- 2.3% and 59.19 +/- 2.36% (mean +/- S.E.M.) inhibition, respectively, whereas empty liposomes (E-LIPO; 250 micrograms lipid/well) of the same lipid composition and free MTX (up to 100 micrograms/well) had no effect on either mediator.

Cite this paper

@article{Williams1995TheEO, title={The effect of liposomally conjugated methotrexate upon mediator release from human peripheral blood monocytes.}, author={Anwen Si{\^a}n Williams and Y L Punn and Nicholas Amos and A C S Mark Cooper and Bryan D. Williams}, journal={British journal of rheumatology}, year={1995}, volume={34 3}, pages={241-5} }