The effect of intestinal distension and of section thickness on counts of epithelial nuclei in the rat small intestine.


(1) A method is described for counting nuclei in wax-embedded histological sections of epithelium. The counts are independent of section thickness over a wide range (4-10 mum). (2) counts of nuclei were made in villi and crypts of rat small intestine fixed either collapsed or distended by a hydrostatic pressure equivalent to 350 mm of water. There was no… (More)