The effect of initial tonicity on freeze/thaw injury to human red cells suspended in solutions of sodium chloride.

@article{Pegg1991TheEO,
  title={The effect of initial tonicity on freeze/thaw injury to human red cells suspended in solutions of sodium chloride.},
  author={David E. Pegg and M. P. Diaper},
  journal={Cryobiology},
  year={1991},
  volume={28 1},
  pages={18-35}
}
Human red blood cells, suspended in solutions of sodium chloride, have been frozen to temperatures between -2 and -14 degrees C and thawed, and the extent of hemolysis was measured. In parallel experiments, red cells were exposed to similar cycles of change in the composition of the suspending solution, but by dialysis at 21 degrees C. The tonicity of the… CONTINUE READING