The effect of histochemical methylation on the phosphate groups of nucleic acids: Interpretation of the absence of nuclear basophilia

@article{Prent1980TheEO,
  title={The effect of histochemical methylation on the phosphate groups of nucleic acids: Interpretation of the absence of nuclear basophilia},
  author={Poul Prent\o},
  journal={The Histochemical Journal},
  year={1980},
  volume={12},
  pages={661-668}
}
The effect of hot methylation (hydrochloric acid-methanol) on nuclear stainability was investigated in order to determine whether the progressive loss of basophilia is due to methylation of the diester phosphate groups of nucleic acid. DNA spots on filter paper were unchanged in their stainability towards Toluidine Blue even after methylation for 4 days, while RNA, chondroitin sulphate and hyaluronic acid lost their affinity for this dye after 4 h methylation. In formalin-fixed sections… CONTINUE READING