The effect of formalin fixation on DNA and the extraction of high-molecular-weight DNA from fixed and embedded tissues.

Abstract

The effect of formalin fixation on DNA and extraction of DNA from fixed tissues was investigated to retrieve archival tissue samples stored in pathology departments for molecular biological studies. Aldehyde fixatives resulted in degradation of DNA at room temperature but not at 4 degrees C. The degradation also occurred in formalin when the pH or the salt… (More)

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@article{Koshiba1993TheEO, title={The effect of formalin fixation on DNA and the extraction of high-molecular-weight DNA from fixed and embedded tissues.}, author={Mamiko Koshiba and K Ogawa and Shuji Hamazaki and Tsuyoshi Sugiyama and Osamu Ogawa and Tsuyoshi Kitajima}, journal={Pathology, research and practice}, year={1993}, volume={189 1}, pages={66-72} }