The effect of folic acid on porphyrin synthesis in tumors and normal skin of mice treated with 5-aminolevulinic acid or methyl 5-aminolevulinate.

  title={The effect of folic acid on porphyrin synthesis in tumors and normal skin of mice treated with 5-aminolevulinic acid or methyl 5-aminolevulinate.},
  author={Li-wei Ma and Arnfinn Engeset Steindal and Asta Juzeniene and Vladimir Iani and Johan Emelian Moan},
  journal={Photochemical \& photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology},
  volume={5 8},
  • Li-wei Ma, A. E. Steindal, +2 authors J. Moan
  • Published 2006
  • Chemistry, Medicine
  • Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
5-Aminolevulinic acid (ALA) or its derivative methyl 5-aminolevulinate (MAL) combined with folic acid was applied in nude mice bearing human colon adenocarcinoma. The aim of the study is to see whether folic acid may increase biosynthesis of porphyrins in tumor tissue after systemic or topical administration of ALA or MAL. The production of porphyrins was determined by spectrofluorometric measurements with an optical fibre probe. It was found that the porphyrin production after i.p injection of… Expand
Photodynamic diagnosis with methyl-5-aminolevulinate in squamous intraepithelial lesions of the vulva: Experimental research
The photodynamic diagnosis using in vivo topical methyl amino-levulinic acid appears to be a promising way to detect the intraepithelial lesions of the vulva through photodiagnosis based on the fluorescence detection of protoporphyrin IX in cancer cells after topical application of a cream of methyl amino. Expand
Post-weaning selenium and folate supplementation affects gene and protein expression and global DNA methylation in mice fed high-fat diets
Adequate levels of selenium and folate post-weaning affected gene expression in colon and liver of offspring, including decreasing Slc2a4 gene expression, and global DNA methylation in liver may be one factor through which the negative effects of a poor diet during early life can be ameliorated. Expand


Production of protoporphyrin IX induced by 5‐aminolevulinic acid in transplanted human colon adenocarcinoma of nude mice can be increased by ultrasound
The results provide a theoretical basis for possible clinical use of ultrasound‐combined ALA or ALA based photodynamic therapy and show that the distribution of PpIX fluorescence in the tumors treated with ultrasound was more homogeneous than that in the tumor given ALA only. Expand
Pharmacology of protoporphyrin IX in nude mice after application of ALA and ALA esters
The distribution of protoporphyrin IX (PpIX) induced in 7 different tissues by these drugs was determined either by spectrofluorometric measurements with an optical fibre probe or by chemical extraction of PpIX from the tissues. Expand
Intravenous injection of ALA led to a more homogeneous distribution of the ALA‐derived porphyrins in the whole noduloulcerative BCC lesions. Expand
5-Aminolevulinic Acid, but not 5-Aminolevulinic Acid Esters, is Transported into Adenocarcinoma Cells by System BETA Transporters
5-ALA, but not 5- ALA methyl ester, was found to inhibit cellular uptake of [3H]-GABA and [14C]-β-alanine, and is transported by β-amino acid and GABA carriers in this cell line. Expand
The effect of dimethylsulfoxide, 1-[2-(decylthio)ethyl]azacyclopentan-2-one and Labrafac(®)CC on porphyrin formation in normal mouse skin during topical application of methyl 5-aminolevulinate: A fluorescence and extraction study.
It is suggested that the new penetration enhancer Labrafac(®)CC in creams with MAL may be used to increase the penetration of MAL through the mouse skin and at the same time increase production of porphyrin in the skin. Expand
Design, synthesis, and biological evaluation of folic acid targeted tetraphenylporphyrin as novel photosensitizers for selective photodynamic therapy.
Targeted PDT offers the opportunity of enhancing photodynamic efficiency by directly targeting diseased cells and tissues by delivering photoactive compounds to KB nasopharyngeal cell line, which is one of the numerous tumor cell types that overexpress folate receptors. Expand
5-Aminolaevulinic Acid Methyl Ester Transport on Amino Acid Carriers in a Human Colon Adenocarcinoma Cell Line¶
The cellular uptake of 5-ALA-ME into WiDr cells seems to be due to active transport mechanisms, involving transporters of nonpolar amino acids. Expand
Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid
The activating effect of folic acid has been observed employing the 35-70% ammonium sulphate precipitated fraction, desalted by dialysis or gel filtration, whereas no action was detected when other partially purified PBG -D preparations were utilized as the enzyme source, suggesting either the presence of sites saturated for the activator, or the existence of a different structural protein conformation. Expand
Ambulant photodynamic therapy of superficial malignomas with 5-ALA in combination with folic acid and use of noncoherent light.
This study reports the first results of ambulant photodynamic treatment with 5-aminolevulinic acid (5-ALA) in combination with folic acid and subsequent illumination with a noncoherent light source, observing a response in all cases, however, in some cases only after the addition of folic Acid. Expand
5‐Aminolevulinic acid‐based photodynamic therapy
Studies have shown that a higher accumulation of ALA‐derived PpIX in rapidly proliferating cells may provide a biologic rationale for clinical use of ALa‐based PDT and diagnosis, however, no review updating the clinical data has appeared so far. Expand