The effect of different divalent cations on the kinetics and fidelity of Bacillus stearothermophilus DNA polymerase

@inproceedings{Vashishtha2018TheEO,
  title={The effect of different divalent cations on the kinetics and fidelity of Bacillus stearothermophilus DNA polymerase},
  author={Ashwani Kumar Vashishtha and William H. Konigsberg},
  booktitle={AIMS biophysics},
  year={2018}
}
Although Mg2+ is the metal ion that functions as the cofactor for DNA polymerases (DNA pols) in vivo, Mn2+ can also serve in this capacity but it reduces base discrimination. Metal ions aside from Mg2+ or Mn2+ can act as cofactors for some DNA pols but not for others. Here we report on the ability of several divalent metal ions to substitute for Mg2+ or Mn2+ with BST DNA polymerase (BST pol), an A family DNA pol. We selected the metal ions based on whether they had previously been shown to be… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 32 REFERENCES

Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2011
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL