The effect of different 3′ untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco

@article{Tangphatsornruang2010TheEO,
  title={The effect of different 3′ untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco},
  author={Sithichoke Tangphatsornruang and Ian Birch-Machin and Christine A. Newell and J. C. Gray},
  journal={Plant Molecular Biology},
  year={2010},
  volume={76},
  pages={385-396}
}
The 3′ untranslated region (3′ UTR) of transcripts is a major determinant of transcript stability in plastids and plays an important role in regulating gene expression. In order to compare the effect of different 3′ UTRs on transgene expression in tobacco chloroplasts, the 3′ UTRs from the tobacco chloroplast rbcL, psbA, petD and rpoA genes and the terminator region of the Escherichia coli rrnB operon were inserted downstream of the gfp reporter gene under the control of the psbA promoter, and… CONTINUE READING

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