The effect of deuterium oxide on the fluorescence of reduced nicotinamide adenine dinucleotide free in solution and in complexes with liver alcohol dehydrogenase.

Abstract

The fluorescence emission intensity at 460 nm of reduced nicotinamide adenine dinucleotide in solution is increased in the presence of deuterium oxide over that observed in water. The increase in fluorescence intensity is a linear function of DzO concentration, and in 75 volume % DzO equals 20%. A similar increase is observed with N… (More)

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