The effect of continuous and interval exercise on PGC‐1α and PDK4 mRNA in type I and type II fibres of human skeletal muscle

  title={The effect of continuous and interval exercise on PGC‐1$\alpha$ and PDK4 mRNA in type I and type II fibres of human skeletal muscle},
  author={L. Wang and Kent Sahlin},
  journal={Acta Physiologica},
Aim:  Differences in fibre‐type recruitment during exercise may induce a heterogenic response in fibre‐type gene expression. We have investigated the effect of two different exercise protocols on the fibre‐type‐specific expression of master genes involved in oxidative metabolism [proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α) and Pyruvate dehydrogenase kinase 4 (PDK4)]. 

Acute upregulation of PGC-1α mRNA correlates with training-induced increases in SDH activity in human skeletal muscle.

It is suggested that acute upregulation of PGC-1α mRNA relates to the magnitude of subsequent training-induced increases in oxidative capacity, but not other molecular and morphological chronic skeletal muscle adaptations.

Exercise-induced cell signalling responses of human skeletal muscle : the effects of reduced carbohydrate availability

The primary aim of this thesis is to characterise the skeletal muscle cell signalling responses thought to regulate mitochondrial biogenesis following an acute bout of high-intensity interval exercise and moderate- intensity continuous exercise.

Adaptation of Skeletal Muscles to Contractile Activity of Varying Duration and Intensity: The Role of PGC-1α

  • D. Popov
  • Biology
    Biochemistry (Moscow)
  • 2018
Investigation of the effects of duration and intensity of aerobic exercise on the PGC-1α-dependent and -independent mechanisms of mitochondrial biogenesis is important for treatment of patients with various metabolic disorders, as for optimization of training in athletes.

The effects of PDK4 inhibition on AMPK protein levels and PGC-1? gene expression following endurance training in skeletal muscle of Wistar rats

It seems that the combination of endurance training and PDK4 inhibition by up-regulation of PGC-1α expression, effectively improves energy state and performance in skeletal muscle.

Effectiveness of Sub-Maximal Intermittent Exercise on Muscle Glycogen Depletion, PGC-1α and PDK-4 gene Expression

It is hypothesized that intermittent exercise performed at high but sub-maximal intensities with long recovery periods would induce a low glycogen state that would stimul- ate peroxisome proliferator-activated receptor-γ coa- ctivator-1α (PGC1-α) and pyruvate dehydrogenase kinase-4 (PDK-4) gene expression in muscle.

Repeatability of exercise‐induced changes in mRNA expression and technical considerations for qPCR analysis in human skeletal muscle

Exercise‐induced changes in mRNA expression are not repeatable even under identical experimental conditions, thereby challenging the use of mRNA expression as a biomarker of adaptive potential and/or individual responsiveness to exercise.

The Effect of Periodic Exercise and Resveratrol Supplementation on the Expression of Pparg Coactivator-1 Alpha and Pyruvate Dehydrogenase Kinase Genes in Gastrocnemius Muscle of Old Rats With Type 2 Diabetes

The combination of resveratrol supplementation and periodic exercise can have beneficial effects on PDK4 and PGC-1α expression levels in the gastrocnemius muscle of old rats with type 2 diabetes and reduce the risks of diabetes-related complications.

Select Skeletal Muscle mRNAs Related to Exercise Adaptation Are Minimally Affected by Different Pre-exercise Meals that Differ in Macronutrient Profile

It is concluded that mRNA quantity associated with muscle adaptation after resistance exercise is not affected by a difference in pre-exercise nutrient availability, and PDK4 mRNA quantity was significantly higher in the FAT condition at 3 h post-resistance exercise compared to CHO.

Transcriptional modulation of mitochondria biogenesis pathway at and above critical speed in mice

Data indicate that at the intensities used in endurance training, the expression of mitochondrial biogenesis genes is finely modulated by the relative intensity of exhaustive exercise.

Training protocols differently affect AMPK-PGC-1α signaling pathway and redox state in trout muscle.



Human skeletal muscle fibre type variations correlate with PPARα, PPARδ and PGC‐1α mRNA

This work investigated expression of those genes implicated in the regulation of oxidative fibre phenotypes in humans that have been instrumental in highlighting genes and their products involved in theregulation of muscle fibre type and oxidative phenotypes from genetically modified animals.

Intensity‐dependent activation of intracellular signalling pathways in skeletal muscle: role of fibre type recruitment during exercise

The authors concluded that the intensity during a single bout of exercise regulates PGC-1α mRNA abundance by activating selected upstream signalling pathways in human skeletal muscle with an intensity-dependent response.

Exercise‐Induced Expression of Vascular Endothelial Growth Factor mRNA in Rat Skeletal Muscle is Dependent on Fibre Type

Exercise‐induced increase in VEGF transcript levels was specifically observed in type IIb myofibres, which are predominantly glycolytic and more susceptible to local hypoxia than oxidative my ofibres such as type I or IIa fibres (110 %, P < 0.05).

Exercise induces transient transcriptional activation of the PGC‐1α gene in human skeletal muscle

It is demonstrated that exercise induces a dramatic transient increase in PGC‐1α transcription and mRNA content in human skeletal muscle, consistent with its role as a transcriptional coactivator, and suggest that PGC•1α may coordinate the activation of metabolic genes in human muscle in response to exercise.

Influence of pre‐exercise muscle glycogen content on exercise‐induced transcriptional regulation of metabolic genes

Low muscle glycogen content enhances the transcriptional activation of some metabolic genes in response to exercise, raising the possibility that signalling mechanisms sensitive to glycogencontent and/or FFA availability may be linked to the transcriptionAL control of exercise‐responsive genes.

Exercise intensity‐dependent regulation of peroxisome proliferator‐activated receptor γ coactivator‐1α mRNA abundance is associated with differential activation of upstream signalling kinases in human skeletal muscle

In conclusion, exercise intensity regulates PGC‐1α mRNA abundance in human skeletal muscle in response to a single bout of exercise, mediated by differential activation of multiple signalling pathways, with ATF‐2 and HDAC phosphorylation proposed as key intensity‐dependent mediators.

Human skeletal muscle fibre type variations correlate with PPAR alpha, PPAR delta and PGC-1 alpha mRNA.

Skeletal muscle mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta reflects differences in type I muscle fibres associated with pathologically and physiologically induced skeletal muscle fibre type differences.

Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression.

Glucose ingestion attenuated the exercise-induced increase in PDK-4 and UCP3 mRNA and PGC-1 mRNA, suggesting that glucose availability can modulate the effect of exercise on metabolic gene expression.

PGC-1α mRNA expression is influenced by metabolic perturbation in exercising human skeletal muscle

A novel finding was that, in human skeletal muscle, PGC-1alpha mRNA increased more after exercise with restricted blood flow than in the nonrestricted condition, which suggests that calcineurin may be activated by exercise in humans and does not exclude that calcinesurin could play a role in P GC-1 transcription activation inhuman skeletal muscle.

Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-gamma coactivator-1 and peroxisome proliferator-activated receptor-alpha in skeletal muscle.

The increases in PGC-1 and PPAR-alpha levels reported in this study may play an important role in the changes in muscle mitochondria content, oxidative phenotype, and sensitivity to insulin known to be induced by endurance training.