The effect of cellular energetics on foreign protein production.

  title={The effect of cellular energetics on foreign protein production.},
  author={Yang-Hsiung Ko and William E. Bentley and William A. Weigand},
  journal={Applied biochemistry and biotechnology},
  volume={50 2},
Escherichia coli strain F-122 was used to determine if there are additional physiological effects, other than decreasing energetic efficiency accompanied by the excretion of the acetate, on foreign protein production. This organism was the host for expressing HIV582-beta-galactosidase fusion protein under the control of the trp promoter, with ampicillin resistance. By comparing parallel batch cultures with and without acetate addition, it was found that the presence of acetate in the media did… CONTINUE READING

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