The effect of cations on C-band formation in human chromosomes.


A number of salt solutions were tested for their ability to replace 2 X SSC in the production of C-bands with the so-called CT technique. Those containing monovalent cations, except for the ones with Ag+ ions, were capable of inducing C-bands in Ba(OH)2-pretreated human chromosomes. Cesium cations were the most effective in this respect. In contrast to the… (More)