The effect of botulinum neurotoxins on the release of insulin from the insulinoma cell lines HIT-15 and RINm5F.

@article{Boyd1995TheEO,
  title={The effect of botulinum neurotoxins on the release of insulin from the insulinoma cell lines HIT-15 and RINm5F.},
  author={Robin H. S. Boyd and Michael Duggan and Clifford Charles Shone and Keith A. Foster},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 31},
  pages={
          18216-8
        }
}
Western blotting of the insulin-secreting beta-cell lines HIT-15 and RINm5F with anti-SNAP-25 (synaptosomal associated protein of 25 kDa), anti-synaptobrevin, and anti-syntaxin 1 antibodies revealed the presence of proteins with the same electrophoretic mobility as found in neural tissue. Permeabilization of both of these insulinoma cell lines to botulinum neurotoxin A by electroporation resulted, after 3 days of culture, in the loss of approximately 90% of SNAP-25 immunoreactivity. A similar… CONTINUE READING

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Towards New Uses of Botulinum Toxin as a Novel Therapeutic Tool

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Truncation of SNAP-25 reduces the stimulatory action of cAMP on rapid exocytosis in insulin-secreting cells.

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