The effect of azide on the spectral and catalytic properties of ascorbate oxidase


(1) 45% of the total copper of green zucchini ascorbate oxidase is EPR-detectable. At least two species of copper are present, one with a small A∥ (Type 1) and one with a large A∥ (Type 2). Computer simulated spectra indicated 50% contribution by each type of copper. (2) Azide inhibited ascorbate oxidase activity by an uncompetitive mechanism. EPR and… (More)
DOI: 10.1007/BF01792080


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