The effect of altering area of application and dose per unit area on sensitization by DNCB

  title={The effect of altering area of application and dose per unit area on sensitization by DNCB},
  author={S. I. White and Peter Simon Friedmann and Celia Moss and Julia Marie Simpson},
  journal={British Journal of Dermatology},
We have investigated the contributions of dose per unit area and area of application to the degree of sensitivity induced by dinitrochlorobenzene (DNCB). Subjects sensitized with 35.4 μg/cm2 showed equal sensitization whether the total dose was 250 μg applied to 7.1 cm2 or 62.5μg applied to 1.8 cm2. Three groups of subjects were sensitized with 16.4 μg/cm2 applied as 56μg on 3.5 cm2, 116μg on 7.1 cm2 or 232μg on 14.2cm2. Although the total dose changed by a factor of four, the proportions of… Expand
The influence of area of application on sensitization by dinitrochlorobenzene
It is suggested that not only is the amount of antigen important in determining response, but also the distribution of the antigen as presented to the afferent limb of the immune system is critical in determining the degree of sensitization. Expand
The relationships between exposure dose and response in induction and elicitation of contact hypersensitivity in humans
It is clear that the dose–response relationships for induction of sensitivity by repeated low‐dose exposures must be carefully defined in future studies. Expand
An evaluation of dose/unit area and time as key factors influencing the elicitation capacity of methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) in MCI/MI‐allergic patients
The study demonstrated that the elicitation threshold for MCI/MI is expected to be in the proximity of 0.025 μg/cm2 although it was not possible to establish a definitive elicitation thresholds in this study. Expand
Low‐dose exposure to antigen induces sub‐clinical sensitization
Responses to a small initial sensitizing dose of antigen were augmented, indicating that sub‐clinical priming of the immune system had indeed occurred. Expand
Thresholds in contact sensitization: immunologic mechanisms and experimental evidence in humans--an overview.
  • M. Boukhman, H. Maibach
  • Medicine
  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2001
The evidence from practical experience in man, from controlled human testing and from both chemical and biological mechanistic considerations, demonstrates that the thresholds for skin sensitizationExpand
Dose metrics in the acquisition of skin sensitization: thresholds and importance of dose per unit area.
The evidence which supports dose per unit area as being the critical exposure metric in the induction of skin sensitization is reviewed, and the mechanistic bases for this relationship are reviewed. Expand
Effect of Prolonged Repeated Exposure to Formaldehyde Donors with Doses Below the EC3 Value on Draining Lymph Node Responses
Results show that repeated and prolonged exposure to doses below the EC3 value can induce reactions above the SI = 3, the hazard identification threshold for sensitization in mice, and one should consider duration of exposure and the possibility of local accumulation of the chemical under investigation. Expand
Contact sensitisation and allergic contact dermatitis: immunobiological mechanisms.
Few Langerhans cells presenting many antigen molecules per cell is a much more potent sensitising stimulus than the same number of molecules presented by many LangerHans cells, each presenting few molecules. Expand
Contact sensitivity to dinitrochlorobenzene is impaired in atopic subjects. Controversy revisited.
If there is a primary dysfunction of the immune system in atopic eczema it might be reflected in altered capacity to generate delayed-type hypersensitivity. Therefore, the dose-response relationshipsExpand
Determination of In Vivo Dose Response and Allergen‐Specific T Cells in Subjects Contact‐Sensitized to Squaric Acid Dibutyl Ester
The in vivo dose‐response characteristics of immune reactivity to SADBE and antigen‐specific T‐cell reactivity are established and established. Expand


Quantitative relationships between sensitizing dose of DNCB and reactivity in normal subjects.
Increase in skinfold thickness proved the best method of assessing response and was linearly related to log challenge dose, and there was a linear relationship between degree of sensitivity and log sensitizing dose so that, on average, each time sensitization dose was halved, the challenge dose required to produce the same response increased 1.5-fold. Expand
Quantitative relationships between sensitising dose
  • 1983