The effect of altering area of application and dose per unit area on sensitization by DNCB

  title={The effect of altering area of application and dose per unit area on sensitization by DNCB},
  author={Stephen I White and Peter Simon Friedmann and Celia Moss and Judy M. Simpson},
  journal={British Journal of Dermatology},
We have investigated the contributions of dose per unit area and area of application to the degree of sensitivity induced by dinitrochlorobenzene (DNCB). Subjects sensitized with 35.4 μg/cm2 showed equal sensitization whether the total dose was 250 μg applied to 7.1 cm2 or 62.5μg applied to 1.8 cm2. Three groups of subjects were sensitized with 16.4 μg/cm2 applied as 56μg on 3.5 cm2, 116μg on 7.1 cm2 or 232μg on 14.2cm2. Although the total dose changed by a factor of four, the proportions of… 

The influence of area of application on sensitization by dinitrochlorobenzene

It is suggested that not only is the amount of antigen important in determining response, but also the distribution of the antigen as presented to the afferent limb of the immune system is critical in determining the degree of sensitization.

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Quantitative relationships between sensitizing dose of DNCB and reactivity in normal subjects.

Increase in skinfold thickness proved the best method of assessing response and was linearly related to log challenge dose, and there was a linear relationship between degree of sensitivity and log sensitizing dose so that, on average, each time sensitization dose was halved, the challenge dose required to produce the same response increased 1.5-fold.

Quantitative relationships between sensitising dose

  • 1983