The effect of additive compounds on glycerol-induced damage to human chondrocytes.

  title={The effect of additive compounds on glycerol-induced damage to human chondrocytes.},
  author={Joshua N Hahn and Leila Laouar and Janet A W Elliott and Gregory S Korbutt and Nadr M Jomha},
High concentrations of cryoprotective agents are required for cryopreservation techniques such as vitrification. Glycerol is a common cryoprotective agent used in cryopreservation protocols but this agent is toxic at high concentrations. This work is an attempt to mitigate the toxic effects of high concentrations of glycerol on intact chondrocytes in human knee articular cartilage from total knee arthroplasty patients by simultaneous exposure to glycerol and a variety of additive compounds. The… Expand
Evaluation of five additives to mitigate toxicity of cryoprotective agents on porcine chondrocytes.
The findings showed that chondroitin sulphate, glucosamine, 2,3,5,6-tetramethylpyrazine and ascorbic acid improved chondrocyte cell survival after exposure to high concentrations of CPAs according to a live-dead cell viability assay. Expand
Antioxidant additives reduce reactive oxygen species production in articular cartilage during exposure to cryoprotective agents.
Results indicated that reactive oxygen species production is induced when articular cartilage is exposed to high CPA concentrations, and correlated with the amount of dead cells. Expand
The effect of cryoprotectant vehicle solution on cartilage cell viability following vitrification.
Evaluating the impact of a subset of vehicle solutions on an established vitrification protocol for articular cartilage found that the normalized cell viability of articular Cartilage vitrified in UCV was significantly greater than that of PBS and may be greater than those of DMEM and X-VIVO. Expand
An electrophysiological comparison of freshly isolated caprine articular chondrocytes versus cryopreserved chondrocytes
Objectives: Cryopreserved chondrocytes find numerous applications in reconstructive surgery, tissue engineering, and cell-based therapy. Cryopreserved chondrocytes may behave differently due to aExpand
Comparison of three multi-cryoprotectant loading protocols for vitrification of porcine articular cartilage.
The optimized protocol proposed based on mathematical modelling resulted in similar chondrocyte recovery to the original protocol and it was 150 min shorter, and this study illustrated the role of CPA permeation (dowel size) and heat transfer (container size) on vitrification protocol outcome. Expand
Vitrification of particulated articular cartilage via calculated protocols
This study developed two 2-step, dual-temperature, multicryoprotectant loading protocols to cryopreserve particulated articular cartilage (cubes) using a mathematical approach, and experimentally measured chondrocyte viability, metabolic activity, cell migration, and matrix productivity after implementing the designed loading protocols, vitrification, and warming. Expand
Vitrification of Intact Porcine Femoral Condyle Allografts Using an Optimized Approach.
Vitrification of femoral condyle allografts can be achieved by the optimized approach and removing the surrounding vitrification solution is advantageous for vitrification outcomes of large size osteochondral allografteds. Expand
Studies on Protection of Astaxanthin from Oxidative Damage Induced by H2O2 in RAW264.7 Cells Based on 1H NMR Metabolomics.
H2O2 treatment, various metabolic pathways such as amino acid, glucose, and glycerolipid metabolism were downregulated which in turn affected citric acid cycle and energy status and AST could reverse downregulation of some of these metabolic pathways to a certain extent, and reduce cellular oxidative stress and death. Expand
Effect of Novel Formulations using Lipophilic Epigallocatechin-3-Gallate against Influenza Virus Infection
Influenza virus infection is associated with high morbidity and mortality, and effective prophylactic and therapeutic methods to reduce infection outbreaks are lacking. Vaccination and currentExpand
Vitamin C and Osteoarthritis Pain-Modulation
Osteoarthritis, a painful irreversible disabling joint disease and one that predominantly affects articular cartilage is rapidly increasing in prevalence among older populations. This overview aimedExpand


Cryoprotective agent toxicity interactions in human articular chondrocytes.
Several CPA solutions are less cytotoxic than single-CPA solutions of the same total concentration and PG was the most toxic CPA when used in combinations. Expand
Cryoprotectant agent toxicity in porcine articular chondrocytes.
This is the most comprehensive study investigating cryoprotectant agent toxicity in articular chondrocytes and has resulted in important information regarding the order of toxicity and interactions that occur between these agents. Expand
Dose-injury relationships for cryoprotective agent injury to human chondrocytes.
The information from this study provides an order of damage to human chondrocytes in situ of commonly used CPAs for vitrification of AC and identifies threshold CPA concentrations for a stepwise loading protocol at which chONDrocyte recovery is significantly decreased. Expand
The Effects of Hypothermic Storage on Chondrocyte Survival and Apoptosis in Human Articular Cartilage
It is hypothesized that the presence of reactive oxygen species, that increase at hypothermic temperatures, could induce apoptosis and lead to the degradation of banked tissue, and distinguish apoptosis from necrosis in human AC after hypthermic storage. Expand
Intramatrix events during cryopreservation of porcine articular cartilage using rapid cooling
  • N. Jomha, P. Anoop, L. Mcgann
  • Chemistry, Medicine
  • Journal of orthopaedic research : official publication of the Orthopaedic Research Society
  • 2004
It appears that successful cryopreservation of viability and function in articular cartilage will require high concentrations of cryoprotectants and rapid cooling. Expand
Mathematical modeling of cryoprotectant addition and removal for the cryopreservation of engineered or natural tissues.
A previously described mathematical model accounting for the mass transfer of CPAs through the sample matrix and cell membrane was expanded to incorporate heat transfer and CPA cytotoxicity, allowing the design of addition and removal protocols that insure CPA equilibration throughout the sample while still minimizing CPA exposure and maximizing cell survival. Expand
Effect of Vitamin C on Mechanical Property of the Regenerated-Cartilage Tissue
Tissue engineering has become a promising option toward the treatment of osteoarthritis, allowing researchers to produce functional replacements for diseased cartilage. The mechanical behavior ofExpand
The protective effect of tetramethylpyrazine on cartilage explants and chondrocytes.
It is demonstrated that T MP has the cartilage and chondroprotective effect, which suggest that TMP could act as an agent for pharmacological intervention in the progress of OA. Expand
Cryopreservation of intact human articular cartilage
It is demonstrated that the cryopreservation procedure used to achieve moderate success with intact sheep AC was not successful with intact human AC and further investigation is required. Expand
Comparison of glucose derivatives effects on cartilage degradation
It is shown that glucosamine derivatives can alter anabolic and catabolic processes in HACs induced by IL-1β, and GlcN-S had the highest chondroprotective activity of all four chemicals. Expand