The effect of active serum albumin on PC12 cells: II. Intracellular Ca2+ transients and their role in neurite retraction.

@article{Dyer1992TheEO,
  title={The effect of active serum albumin on PC12 cells: II. Intracellular Ca2+ transients and their role in neurite retraction.},
  author={David Dyer and Gabor J Tigyi and Ricardo Miledi},
  journal={Brain research. Molecular brain research},
  year={1992},
  volume={14 4},
  pages={302-9}
}
In the preceding paper it was shown that an isoform of serum albumin (ASA; active serum albumin) causes a rapid retraction of neurites and increases intracellular content of Ins1,4,5P3 in PC12 cells. Here we examined whether ASA's effects in nerve growth factor-differentiated PC12 cells were mediated through the Ins1,4,5P3/Ca2+ second messenger pathway by monitoring intracellular Ca2+ (Ca2+i) with Fura2. It was found that ASA caused a dose-dependent increase in Ca2+i. In Ca(2+)-free medium, the… CONTINUE READING