The effect of acceptor oligoribonucleotide sequence on the T4 RNA ligase reaction.

@article{Romaniuk1982TheEO,
  title={The effect of acceptor oligoribonucleotide sequence on the T4 RNA ligase reaction.},
  author={E Romaniuk and Larry W. McLaughlin and Tracy Neilson and Paul J. Romaniuk},
  journal={European journal of biochemistry},
  year={1982},
  volume={125 3},
  pages={639-43}
}
In order to make efficient use of T4 RNA ligase in a program involving chemical-enzymatic oligoribonucleotide synthesis, the sequence effects of the acceptor oligomer have been refined. The reaction of the donor molecules pCp and pUpUpUpCp with a series of trinucleoside diphosphates ApApN, CpNpA and NpCpA (where N = U, C, A or G) was examined. High… CONTINUE READING