The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus).

Abstract

AIM To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). METHODS AND RESULTS Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 10(7) CFU g(-1) for 32 days. Ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici with an endogenous uncultured bacterium during a period of reverting to nonsupplemented feeding. Light microscopy revealed that gut integrity and leucocyte levels were unaffected by Ped. acidilactici; however, blood leucocyte levels and serum lysozyme activity were elevated after 14-days' feeding. No significant improvements in growth performance were observed at the end of the trial (day 32), but survival was significantly higher in the probiotic group. CONCLUSIONS   The study demonstrates that oral supplementation of Ped. acidilactici modulates intestinal bacterial communities in on-growing red tilapia and also stimulates some aspects of the nonspecific immune response. SIGNIFICANCE AND IMPACT OF THE STUDY   To our knowledge this is the first study assessing the effects of probiotics on the gut microbiota of tilapia using culture-independent methods. Such methods are crucial to understand the mechanisms which underpin and mediate host benefits.

DOI: 10.1111/j.1365-2672.2010.04713.x
010020020102011201220132014201520162017
Citations per Year

532 Citations

Semantic Scholar estimates that this publication has 532 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Ferguson2010TheEO, title={The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus).}, author={Roger Ferguson and Daniel Lee Merrifield and G Michael Harper and M D Rawling and Shyamoli Mustafa and Simona Picchietti and Jos{\'e} Luis Balc{\'a}zar and Sean J Davies}, journal={Journal of applied microbiology}, year={2010}, volume={109 3}, pages={851-62} }