The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes.

@article{Kwon2007TheEO,
  title={The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes.},
  author={Y-D Kwon and O-H Kwon and H-S Lee and P. Y. Kim},
  journal={Journal of applied microbiology},
  year={2007},
  volume={103 6},
  pages={2340-5}
}
AIMS To understand the modification of C4-metabolism under anaerobic glycolysis condition by overexpressing anaplerotic enzymes, which mediating carboxylation of C3 into C4 metabolites, in Escherichia coli. METHODS AND RESULTS Anaplerotic NADP-dependent malic enzyme (MaeB), as well as the other anaplerotic enzymes, including phosphoenolpyruvate carboxylase (Ppc), phosphoenolpyruvate carboxykinase (Pck) and NAD-dependent malic enzyme (MaeA), were artificially expressed and their C4 metabolism… CONTINUE READING

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