The effect of DMSO on natural DNA conformation in enhancing transcription.

@article{Juang1987TheEO,
  title={The effect of DMSO on natural DNA conformation in enhancing transcription.},
  author={Jyh-Ching Juang and Hung Jen Liu},
  journal={Biochemical and biophysical research communications},
  year={1987},
  volume={146 3},
  pages={1458-64}
}
In the presence of 5% dimethylsulfoxide, 2.5 fold increase in vitro RNA synthesis rate by E. coli RNA polymerase was observed as superhelical pBR322 DNA was used as template. The effect of DMSO on DNA conformation was studied by: [i] measuring the dissociation constant of EtBr-DNA complex and the maximum EtBr binding site of EtBr in DNA which changed from 7.52 X 10(-7) M and 0.18 site per nucleotide to 9.61 X 10(-7) M and 0.156 site per nucleotide respectively when 10% DMSO was supplemented… CONTINUE READING