The ecologist's field guide to sequence‐based identification of biodiversity

  title={The ecologist's field guide to sequence‐based identification of biodiversity},
  author={Simon Creer and Kristy Deiner and Serita D. Frey and Dorota L. Porazinska and Pierre Taberlet and W. Kelley Thomas and Caitlin Potter and Holly M. Bik},
  journal={Methods in Ecology and Evolution},
The past 100 years of ecological research has seen substantial progress in understanding the natural world and likely effects of change, whether natural or anthropogenic. Traditional ecological approaches underpin such advances, but would additionally benefit from recent developments in the sequence‐based quantification of biodiversity from the fields of molecular ecology and genomics. By building on a long and rich history of molecular taxonomy and taking advantage of the new generation of DNA… 

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Encouraging results suggest that the rapid uptake of DNA-based approaches is supported by a bioinformatic infrastructure capable of assessing both the losses to biodiversity caused by global change and the effectiveness of conservation efforts aimed at slowing or reversing these losses.

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It is argued that it is essential to integrate conventional monitoring methods with novel DNA methods, to validate methods, and to better use and interpret data to improve biodiversity assessment and conservation planning and land management decisions.

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Novel metabarcoding protocols based on the use of short fragments of 18S rRNA or cytochrome c oxidase I genes as genetic markers are presented, allowing characterization of communities living on hard-bottom substrates or other marine benthic ecosystems.

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This review focuses on studies using DNA data for taxonomy, and for environmental studies through metabarcoding on freshwater meiofauna, and reveals that the use of DNA data is still rather limited.

Uses and Misuses of Environmental DNA in Biodiversity Science and Conservation

The study of environmental DNA (eDNA) has the potential to revolutionize biodiversity science and conservation action by enabling the census of species on a global scale in near real time. To achieve

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Comparison of morphological, DNA barcoding, and metabarcoding characterizations of freshwater nematode communities

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Metabarcoding From Microbes to Mammals: Comprehensive Bioassessment on a Global Scale

Global biodiversity loss is unprecedented, and threats to existing biodiversity are growing. Given pervasive global change, a major challenge facing resource managers is a lack of scalable tools to

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Molecular detection of trophic interactions: emerging trends, distinct advantages, significant considerations and conservation applications

  • E. Clare
  • Environmental Science, Biology
    Evolutionary applications
  • 2014
Emerging patterns that have come from the various initial model systems, the advantages and limitations of the technique and key areas where these methods may significantly advance the empirical and applied conservation practices are described.

Systems biology for ecology : from molecules to ecosystems

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