The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo.

@article{Fekete2005TheEC,
  title={The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo.},
  author={Christie A Fekete and Drew J Applefield and Stephen A. Blakely and Nikolay E Shirokikh and Tatyana V. Pestova and Jon R. Lorsch and Alan G Hinnebusch},
  journal={The EMBO journal},
  year={2005},
  volume={24 20},
  pages={3588-601}
}
Translation initiation factor 1A stimulates 40S-binding of the eukaryotic initiation factor 2 (eIF2)/GTP/Met-tRNA(iMet) ternary complex (TC) and promotes scanning in vitro. eIF1A contains an OB-fold present in bacterial IF1 plus N- and C-terminal extensions. Truncating the C-terminus (deltaC) or mutating OB-fold residues (66-70) of eIF1A reduced general translation in vivo but increased GCN4 translation (Gcd- phenotype) in a manner suppressed by overexpressing TC. Consistent with this, both… CONTINUE READING

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