The dynamics of individual nucleosomes controls the chromatin condensation pathway: direct atomic force microscopy visualization of variant chromatin.

@article{Montel2009TheDO,
  title={The dynamics of individual nucleosomes controls the chromatin condensation pathway: direct atomic force microscopy visualization of variant chromatin.},
  author={Fabien Montel and Herv{\'e} Menoni and Martin Castelnovo and J. Bee Bednar and Stefan Dimitrov and Dimitar Angelov and Cendrine Faivre-Moskalenko},
  journal={Biophysical journal},
  year={2009},
  volume={97 2},
  pages={544-53}
}
Chromatin organization and dynamics is studied at scales ranging from single nucleosome to nucleosomal array by using a unique combination of biochemical assays, single molecule imaging technique, and numerical modeling. We show that a subtle modification in the nucleosome structure induced by the histone variant H2A.Bbd drastically modifies the higher order organization of the nucleosomal arrays. Importantly, as directly visualized by atomic force microscopy, conventional H2A nucleosomal… CONTINUE READING