The dynamic three-dimensional organization of the diploid yeast genome

Abstract

The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. However, even in this well-studied model, it is unclear how homolog pairing in diploids or environmental conditions influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation using a polymer model, we observe significant homolog proximity that increases in saturated culture conditions. Surprisingly, we observe a localized increase in homologous interactions between the HAS1-TDA1 alleles specifically under galactose induction and saturated growth. This pairing is accompanied by relocalization to the nuclear periphery and requires Nup2, suggesting a role for nuclear pore complexes. Together, these results reveal that the diploid yeast genome has a dynamic and complex 3D organization.

DOI: 10.7554/eLife.23623

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Cite this paper

@inproceedings{Kim2017TheDT, title={The dynamic three-dimensional organization of the diploid yeast genome}, author={Seungsoo Kim and Ivan Liachko and Donna Garvey Brickner and Kate Cook and William S. Noble and Jason H Brickner and Jay Shendure and Maitreya J. Dunham}, booktitle={eLife}, year={2017} }